-
- Application
- Flow Cytometry (FACS), Activation (Act)
- Purpose
- Cell Activation Cocktail (with Brefeldin A) is composed of PMA, ionomycin, and Brefeldin A.
- Characteristics
- Cell Activation Cocktail (with Brefeldin A) is a pre-mixed cocktail with optimized concentration of PMA (phorbol 12-myristate-13-acetate), ionomycin, and protein transport inhibitor (Brefeldin A). Activation of cells with PMA and ionomycin leads to cytokine production that is captured in the rough endoplasmic reticulum and Golgi apparatus by the protein transport inhibitor Brefeldin A. Brefeldin A functions to inhibit intracellular protein transport, thus allowing intracellular detection of the secreted cytokine and proteins by immunofluorescent staining.
- Components
- Cell Activation Cocktail (with Brefeldin A)
- Solubility
- DMSO
-
-
- Comment
-
Each vial of this cocktail contains phorbol-12-myristate 13-acetate (40.5 µM), ionomycin (669.3 µM), and Brefeldin A (2.5 mg/ml) in DMSO (500X).
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- Each vial of this cocktail contains phorbol-12-myristate 13-acetate (40.5 μM), ionomycin (669.3 μM), and Brefeldin A (2.5 mg/mL) in DMSO (500X).
- Handling Advice
- Protect from light. Avoid repeat freeze/thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store cocktail at -70°C upon receipt.
-
You are here:
