SARS-CoV-2 Spike protein RBD-coupled magnetic beads

Catalog No. ABIN6952716

Product Details

Target: SARS-CoV-2 Spike S1

Binding Specificity: RBD

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Application: Flow Cytometry (FACS), Stimulation (St)

Purpose: SARS-CoV-2 Spike protein RBD-coupled magnetic beads

Characteristics: The pre-coupled magnetic beads coupled with biotinylated SARS-CoV-2 Spike RBD protein to streptavidin conjugated magnetic beads, which can capture the Anti- SARS-CoV-2 antibody or ACE2 protein from cell or serum sample.

The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results.

This very first SARS-CoV-2 Spike protein RBD-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready to use products could greatly save your time and hassle.

Sterility: 0.22 μm filtered

Components: Coupled amount of S1 protein: 80 μg / 2 mg beads

Affinity: Capture ability of Antibody/Sample: > 200 nmol / mg Beads

Application Details

Application Notes: This product is intended for immunocapture, cell stimulating, biopanning and flow cytometry.

Comment:

1. Reconstitute the Beads following the COA, wash the Beads and suspended to a certain concentration by adding assay Buffer.
2. Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.

• Cell stimulating: In order to study some downstream mechanisms, this antigen coupled beads can be used to stimulate your target cells. It can be easily removed using a magnet when its work is done.
• Biopanning: Biopanning is an affinity selection technique which selects for peptides that binds to a given target. In the capturing step, just add the antigen couple beads to your phage library. Simply use a magnet to separate the bound phages from the unbound ones. More efficient and time saving compared to the plate based capture.
• Flow cytometric analysis: This antigen coupled beads can be used as feeder cells (antigen-presenting cells) to run your flow cytometric analysis, but without the hassle of cell culture.

Protocol: The Antigen pre Coupled magnetic beads are Coupled with Biotinylated protein onto streptavidin (SA) magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 Mol/L, the Biotinylated protein can bind to the SA beads irreversibly. We provide the SARS-CoV-2 Spike protein RBD-coupled magnetic beads, which could help you to capture the antibody or ACE-2 protein, and easily to follow up with other tests, such as Cell stimulating, biopanning or Flow cytometric analysis.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Ultrapure water

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 0.05 % Tween-20, pH 7.4, with 10 % Trehalose

Handling Advice: Do not to freeze thaw the Beads after reconstitution.

Storage: -20 °C

Storage Comment: Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state. Once the Beads reconstitution, please use it immediately.

References

Greaney, Loes, Gentles, Crawford, Starr, Malone, Chu, Bloom: "Antibodies elicited by mRNA-1273 vaccination bind more broadly to the receptor binding domain than do those from SARS-CoV-2 infection." in: Science translational medicine, (2021) (PubMed).

Garrett, Galloway, Chu, Itell, Stoddard, Wolf, Logue, McDonald, Matsen, Overbaugh: "High resolution profiling of pathways of escape for SARS-CoV-2 spike-binding antibodies." in: bioRxiv : the preprint server for biology, (2020) (PubMed).

Target Details

Target: SARS-CoV-2 Spike S1

Abstract: SARS-CoV-2 Spike S1 Products

Synonyms: E2 Accessory Reagents, Surface Glycoprotein Accessory Reagents, S Accessory Reagents

Target Type: Viral Protein

Gene ID: 43740568

UniProt: P0DTC2