CUT&RUN Concanavalin A Beads

Catalog No. ABIN6923139

Product Details

Application: Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Separation (Sep)

Purpose: CUT&RUN Concanavalin A Beads of our CUT&RUN Sets.

Specificity: The unique saccharide-binding properties of plant lectins, such as Concanavalin A have made them useful for the labeling and isolation of glycan-presenting cells and glycoproteins in serum and cell lysate.

Characteristics: Concanavalin A is covalently attached to functionalized Magnetic iron oxide particles for use in glycoprotein isolations from serum and cellular extracts.

Components: Concanavalin A coated particles

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

• Metal ions (calcium and manganese) mediate the binding to Concanavalin A and stabilize is conformation.
• The use of buffers with EDTA or other metal chelators must be avoided as it will result in a loss of carbohydrate binding ability.

Reagent Preparation:

Prepare CUT&RUN Concanavalin A Beads

• Pipette 10 µL CUT&RUN Concanavalin A Beads slurry for each sample into a tube
• Place the tubes on a magnet separator and remove the liquid carefully
• Remove the tubes from the magnetic separator
• Wash beads 3 more times with 1 mL Binding Buffer
• Finally resuspend the beads with 10 µL Binding Buffer per sample

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5 μg/μL

Buffer: Stored in 10 mM PBS with 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze the product!
Vortex bead suspension well before use.

Storage: 4 °C