Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

TrueBlot® Anti-Mouse IgG Magnetic Beads

Catalog No. ABIN6809833
  • Application
    Immunoprecipitation (IP), Western Blotting (WB)
    Purpose
    TrueBlot® goat Anti-mouse IgG magnetic beads can be used for separation and purification of mouse antibodies from serum or mouse antibody-labeled components, as well as for immunoassays, immunoprecipitation, and IP Western blots.
    Brand
    TrueBlot®
    Characteristics
    TrueBlot® Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The super-paramagnetic nanoparticles are coupled with a biomolecule, such as goat Anti-mouse IgG, and are specifically designed, tested and quality controlled for isolation and purification of mouse IgG, and immunoprecipitation methods using manual or automatic platforms. This antibody binds the heavy chain of mouse IgG and is suitable for immunoassays that utilize a mouse IgG primary polyclonal antibody. Cell separation and sorting can be achieved using a mouse IgG antibody to defined cell surface antigens. The beads have a large surface area with high capture efficiencies. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume. Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL.
  • Application Notes
    Western Blot : User Optimized
    ImmunoPrecipitation: User Optimized
    Other Dilution: User Optimized
    Comment

    TrueBlot® goat Anti-mouse IgG magnetic beads can be used for separation and purification of mouse antibodies from serum or mouse antibody-labeled components, as well as for immunoassays, immunoprecipitation, and IP Western blots. For antibody purification, goat Anti-mouse IgG magnetic beads are incubated with the mouse antibody solution and then separated by magnets. After the unbound particulates are washed from the beads, the bound antibodies are eluted from the beads using the elution buffer. The beads are then magnetically separated from the eluted solution, which is removed manually. For IP, target specific antibody is incubated with goat Anti-mouse IgG magnetic beads. The unbound antibody is washed and the sample containing target antigen is added. After unbound particulates are washed from the beads, the purified protein is eluted from the beads using elution buffer. The samples are then resolved by SDS-PAGE and analyzed by Western blotting.

    Restrictions
    For Research Use only
  • Buffer
    0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
    Stabilizer: None
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store vial at 4 °C prior to opening. DO NOT FREEZE.
You are here:
Blank Blank Blank Blank
Blank Blank Blank Blank
Support