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- Reactivity
- Mouse
- Host
- Rat
- Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS)
- Characteristics
- In mice, CD4+ T cells can be divided into naive and memory cells based on expression of adhesion molecules CD44 and CD62L. Naive T cells exhibit high levels of CD62L and low expression of CD44, whereas memory T cells are identified by high CD44 and low CD62L expression. This panel includes antibodies against CD3, CD4, CD62L and CD44, which can be used together to identify naive and memory T cell subsets in mice. Alterations in these subsets are evident in older mice following antigenic stimulation.
- Components
- Mouse Naive/Memory T cell ID Panel, Rat IgG2b/Rat IgG2a/Rat IgG2a/Rat IgG2b
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- Application Notes
- This reagent contains one vial each of APC/Cy7 conjugated anti-CD3 (clone 17A2), PerCP/Cy5.5 conjugated anti-CD4 (clone RM4-5), APC conjugated anti-CD62L (clone MEL-14), and PE conjugated anti-CD44 (clone IM7) at optimal concentration for four-color flow cytometric analysis.
- Restrictions
- For Research Use only
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- Buffer
- Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from prolonged exposure to light. Do not freeze.
- Storage
- 4 °C
- Storage Comment
- The antibody solution should be stored undiluted between 2°C and 8°C.
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