Anti-DYKDDDDK G1 Affinity Resin
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- Target See all DYKDDDDK Tag products
- DYKDDDDK Tag
- Reactivity
- Tag
- Clonality
- Monoclonal
- Application
- Immunoprecipitation (IP), Purification (Purif), Separation (Sep)
- Specificity
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High DYKDDDDK-tag binding capacity and ultralow levels of non-specific binding
Multiple elution methods for diverse protein characteristics and different requirements
Used for purification and immunoprecipitation of DYKDDDDK-tagged protein or protein complex - Characteristics
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DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. Anti-DYKDDDDK G1 Affinity Resin is designed for the purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK-tagged protein in the cell lysate can specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate.
Matrix: 4 % cross-linked agarose
Average bead size: 90 μm
Ligand: Mouse monoclonal antibody against DYKDDDDK tag, clone G1
Binding capacity: Approximately 1 mg DYKDDDDK-tagged protein per ml settled resin
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- Comment
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Resin reuse: The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.
Elution method: Acid, alkaline, neutral, peptide competitive and SDS-PAGE loading buffer elutions
Reagents compatibility: Compatible with commonly used reagents at certain concentrations - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 50 % settled resin in TBS with 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze the resin.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for up to 12 months.
- Expiry Date
- 12 months
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- Target
- DYKDDDDK Tag
- Alternative Name
- DYKDDDDK (DYKDDDDK Tag Products)
- Target Type
- Tag
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