Streptavidin-HRP

Catalog No. ABIN1536533

Product Details

Application: Hybridoma Screening (HS), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Northern Blotting (NB), Immunohistochemistry (IHC), Southern Blotting (SB), Western Blotting (WB)

Application Details

Application Notes: Working concentrations for specific applications should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time. The following dilution ranges are recommended starting points for this product: Dot blot: 1:2,000-1:10,000 Western blot: 1:2,000-1:20,000 ELISA: 1:50,000-1:200,000 Others: User-optimized

Restrictions: For Research Use only

Handling

Concentration: 1 mg/ml

Buffer: PBS (pH 7.4) containing 10 mg/ml BSA and 0.01% thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The reagent may be stored at 4°C for short periods of one month or less. For longer periods,aliquot and store at -20°C . Avoid repeated freeze/thaw cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use.

References

Metzner, Luckert, Lemuth, Hämmerle, Moos: "Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection." in: Sensors (Basel, Switzerland), Vol. 18, Issue 5, (2018) (PubMed).

Jacobson, MacFadden, Wu, Peng, Liu: "Autoregulation of the 26S proteasome by in situ ubiquitination." in: Molecular biology of the cell, Vol. 25, Issue 12, pp. 1824-35, (2014) (PubMed).

Dreyer, Matile, Papastogiannidis, Kamber, Favuzza, Voss, Wittlin, Pluschke: "Passive immunoprotection of Plasmodium falciparum-infected mice designates the CyRPA as candidate malaria vaccine antigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 12, pp. 6225-37, (2012) (PubMed).

Wang, Wu, Hong, Chen, Xu, Niu, Peng, Liu, Wang, Liu, Agrez, Niu: "PKC promotes the migration of colon cancer cells by regulating the internalization and recycling of integrin ?v?6." in: Cancer letters, Vol. 311, Issue 1, pp. 38-47, (2011) (PubMed).

Li, Peng, Hu, Xu, Xie, Yuan: "Localized expression pattern of miR-184 in Drosophila." in: Molecular biology reports, Vol. 38, Issue 1, pp. 355-8, (2010) (PubMed).

Target Details

Background: Streptavidin is a protein that has similar binding properties to avidin. It is isolated from Streptomyces avidinii. Streptavidin has a molecular weight of 60 KD and four subunits. Each subunit can bind one molecule of biotin. Biotin is a 244-dalton water-soluble vitamin. Streptavidin has an extremely high binding affinity: (Kd=10-15) for biotin.Streptavidin-HRP is steptavidin coupled to horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary and secondary antibodies in enzyme immunoassays, immunoblotting, immunohistochemistry, flow cytometry,and hybridoma screening systems. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern and northern blots.