(2 references)-
- Target See all MAPK14 products
- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
- Binding Specificity
- pThr180, pTyr182
- Reactivity
- Human, Mouse, Rat
- Application
- Immunoassay (IA), Flow Cytometry (FACS)
- Brand
- BD™ Cytometric Bead Array,BD™
- Characteristics
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The BD™ CBA Phospho p38 (T180/Y182) Flex Set is a bead-based immunoassay capable of measuring human, mouse, or rat p38, a member of the mitogen-activated protein kinase (MAPK) family of kinases, that has been threonine-phosphorylated at Thr-180 and tyrosine-phosphorylated at Tyr-182 in denatured cell lysate samples. Human and mouse reactivity was determined by testing cell lysates with the BD CBA Phospho p38 (T180/Y182) Flex Set. Reactivity with rat samples was determined by western blot for each of the antibodies used in the BD CBA Phospho p38 (T180/Y182) Flex Set. The biology and function of p38 has been previously reviewed. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005 or 560006).
This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
The Phospho p38 (T180/Y182) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 ml microfuge tube for reconstitution. Reconstitute the standard with 0.1 ml Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/ml and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use. - Components
- Capture beads, detection reagent, standards
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- Comment
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Bead Position: B6
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
- Buffer
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light. The Phospho p38 (T180/Y182) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
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- Target
- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
- Alternative Name
- p38 (MAPK14 Products)
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