Julian Pampel, BSc
Creative mind of antibodies-online with a keen eye for details. Proficient in the field of life-science with a passion for plant biotechnology and clinical study design. Responsible for illustrated and written content at antibodies-online as well as supervision of the antibodies-online scholarship program.
Articles by Julian Pampel
Cytokeratins in the detection of tumors
Cytokeratins (CKs, or following more recent nomenclature also simply called keratins) are intermediate filament forming proteins that provide mechanical support and fulfill a variety of additional functions in epithelial cells. Read more
anti-Immunoglobulin G (IgG) secondary antibodies
Each IgG is composed of four peptide chains - two class γ heavy chains of about 50 kDa and two light chains of about 25 kDa - with two antigen binding sites. Hence, IgG antibodies are large molecules of about 150 kDa. Read more
anti-Immunoglobulin A (IgA) secondary antibodies
Immunoglobulin A plays a role in mucosal immunity and is produced in mucosal linings. Hence IgA can be found in mucous secretions, including tears, saliva, colostrum, secretions from the gastrointestinal tract, and others, but it is found only in small amounts in blood. Read more
anti-Immunoglobulin E (IgE) secondary antibodies
IgE is only found in mammals. IgE is the least abundant isotype of all antibodies, but it is capable of triggering the most powerful immune reactions. IgE plays an important role in allergy, and leads to excessive activation of mast cells and basophils by binding to their Fc receptors, resulting in an extreme inflammatory response. Read more
anti-Immunoglobulin M (IgM) secondary antibodies
IgM is constructed of five or six units (i.e. mostly as pentamers but also hexamers occur) which are each comprised of two heavy-chains (mu-chains) and two light chains, bound together by disulfide bonds and a so-called J-chain. Read more
anti-Immunoglobulin Y (IgY) secondary antibodies
Immunoglobulin Y (IgY) is found in birds, and it is being isolated for biotechnological applications from egg-yolk of chicken eggs. Studies have suggested that Immunoglobulin Y is functionally similar to IgG, and also shares some similarity with IgE. Read more
anti-Immunoglobulin D (IgD) Secondary Antibodies
IgD (IgD) is a monomeric antibody isotype that is expressed in the plasma membranes of immature B-lymphocytes. IgD is also produced in a secreted form that is found in small amounts in blood serum. Secreted IgD is made up of two heavy chains of the delta class, and two light chains. IgD's function has long been a conundrum and is still incompletely understood. IgD is present in species ranging from fish to human (probably with the exception of birds), indicating that IgD has been preserved since its ancient origins which suggests that IgD has important immunological functions. Read more
What is Flow Cytometry (FACS Analysis)?
Originally developed in the late 1960s, flow cytometry is a popular analytical cell-biology technique that utilizes light to count and profile cells in a heterogenous fluid mixture. Flow cytometry is a particularly powerful. Read more
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) is a method that allows detection of proteins or other antigens in tissue sections. For this purpose the sections are exposed to labelled antibodies that are directed against epitopes in the target protein. A target can thereby be visualized by a marker such as fluorescent Read more
Enzyme-linked immunosorbent assay (ELISA)
Enzyme-linked Immunosorbent assay (ELISA) can provide a useful measurement of antigen or antibody concentration. Read more
Peptide Synthesis - Methods and Reagents
In organic chemistry, peptide synthesis is the production of peptides, in which multiple amino acids are linked via peptide bonds. In contrast the biological process of producing long peptides (proteins) is usually referred to as protein biosynthesis. Read more
Magnetic Beads
Magnetic beads are an ideal tool for immobilizing molecules like proteins (enzymes, antibodies, peptides etc.) or nucleic acids on a solid phase; they can be further used in various applications. Read more
Independent Validation Initiative
Most commercially available antibodies lack critical validation data. This is why we started the (IVI) to independently validate commercialantibodies and ELISA kits. The Independent Validation Initiative is centered around core values of transparency, reproducibility, scientific integrity. Read more
Products for Plant-specific Targets
Modern molecular biology has opened up a whole new range of techniques and possibilities to scientists working in the different fields of the classical plant sciences like plant physiology, breeding or pathology. Read more
Protein Labels
Biological research often requires the use of molecular labels that are covalently attached to a protein of interest to facilitate detection or purification of the labeled protein and/or its binding partners. Read more
Protein Expression Systems
Comparison of Recombinant Protein Expression Systems: Advantages and downfalls of E.coli, Yeast, CHO and plant cells in recombinant protein production. Discover high quality proteins from antibodies-online. Read more
Model Organisms
When researchers look for an organism to use in their studies, they look for several traits. Among these are size, generation time, accessibility, manipulation, genetics, conservation of mechanisms, and potential economic benefit. Read more
Chromatin immunoprecipitation (ChIP)
Immunoprecipitation of protein-DNA complexes (ChIP) enables the study of epigenetic relations. The technique identifies binding sites of DNA-associated proteins and allows a closer study of transcription factors. Read more
CUT&RUN - Improved Method to Study Protein-DNA Interactions
CUT&RUN-sequencing, also known as Cleavage Under Targets and Release Using Nuclease, is a method used to analyze protein interactions with DNA. CUT&RUN-sequencing combines antibody-targeted controlled cleavage by micrococcal nuclease with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. Read more
SARS-CoV-2 Life Cycle: Stages and Inhibition Targets
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the enveloped positive-sense RNA viruses. This virus is characterized by club-like spikes on the surface, and a unique replication strategy. Cell entry of coronaviruses depends on binding of the viral spike (S) proteins to cellular receptors and on S protein priming by host cell proteases. Unravelling which cellular factors are used by SARS-CoV-2 for entry might provide insights into viral transmission and reveal therapeutic targets. Read more
antibodies-online CUT&RUN and CUT&Tag Handbook
antibodies-online CUT&RUN and CUT&Tag Handbook Read more
GFP Catcher - High-Affinity Single-Domain Antibodies
antibodies-online offers catchers for / ideal tool. Our current product lines are based on recombinant single-domain antibodies (sdAbs) derived from llamas or alpacas. This type of affinity molecules (also known as “Nanobodies”; trademark owned by Ablynx) provide significant advantages over conventional IgG molecules. All of our products are produced in-house. They are highly specific with affinities tailored for a wide range of applications. Read more
CUT&RUN Combo: Beads, Controls, Primary, MNase in One go!
CUT&RUN stands for "Cleavage Under Targets and Release Using Nuclease". CUT&RUN introduces some major modifications to eliminate shortcomings inherent to ChIP-seq. Benefit from our customizable CUT&RUN Combo : Choose from validated Beads, a wide collection of primary Antibodies, Controls, and pAG-MNase Read more
Hallmarks of Cancer Handbook
Download our Hallmarks of Cancer Handbook Read more
CRISPR-Cas9 System
Neurodegenerative Diseases
Huntington's Disease
SARS-CoV-2 Proteins
antibodies-online has served hundreds of research institutions around the world with SARS-CoV-2 Proteins and SARS-CoV-2 Recombinant Proteins. If you are looking for the right SARS-CoV-2 Protein to suit your research setting, for Immunizations, for Kit Dev Read more
SARS-CoV-2 / COVID-19 ELISA Kits
antibodies-online has served hundreds of research institutions around the world with SARS-CoV-2 ELISA Kits. If you are looking for the right SARS-CoV-2 ELISA Kit to suit your research setting, we have grouped our current SARS-CoV-2 ELISA Kit catalogue by Read more
Protein Tags
Hallmarks of Cancer
Hallmarks of Cancer Read more
Epigenetics
Histone H3 Antibodies
Histone H3 Antibodies Read more
Histone H4 Antibodies
Histone H4 Antibodies Read more
Transcription Factors
Transcription Factors Read more
CUT&Tag
CUT&Tag Read more
DNA Modification
DNA Modification Read more
Chromatin Remodeling
Chromatin Remodeling Read more
Immuno-Oncology
Cytokeratins in Tumor Detection
Host Cell Proteins
Host Cell Proteins Read more
Lateral Flow Antibodies
Lateral Flow Antibodies Read more
RNA Based Therapeutics
RNA Based Therapeutics - Drug Discovery Read more
Spatial Biology
Spatial Biology Read more
Biosimilar Antibodies
The Role of Fc Receptor Neonatal in Cancer
Autoimmune Diseases
Autoimmune Diseases Read more
Multiple Sclerosis
Multiple Sclerosis Read more
