AadA1 antibody
aadA1
Reactivity: Chlamydomonas reinhardtii, Nicotiana tabacum
WB
Host: Rabbit
Polyclonal
unconjugated
1 image
(1 reference)-
- Target
- AadA1 (aadA1) (Aminoglycoside adenyltransferase (aadA1))
- Reactivity
- Chlamydomonas reinhardtii, Nicotiana tabacum
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Specificity
- AadA cassette in Nicotiana tabacum.
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- aadA cassette
- Characteristics
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Expected / apparent Molecular Weight of the Antigene: 30 / 30 kDa
chloroplast transformation marker - Purification
- serum
- Immunogen
- KLH-conjugated peptide derived from known aadA1 sequences
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- Application Notes
- 1: 3 000 with standard ECL (WB)
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 μL of sterile water
- Handling Advice
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
- Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "Optimization of transplastomic production of hemicellulases in tobacco: effects of expression cassette configuration and tobacco cultivar used as production platform on recombinant protein yields." in: Biotechnology for biofuels, Vol. 6, Issue 1, pp. 65, (2013) (PubMed).
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: "Optimization of transplastomic production of hemicellulases in tobacco: effects of expression cassette configuration and tobacco cultivar used as production platform on recombinant protein yields." in: Biotechnology for biofuels, Vol. 6, Issue 1, pp. 65, (2013) (PubMed).
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- Target
- AadA1 (aadA1) (Aminoglycoside adenyltransferase (aadA1))
- Background
- AadA - aminoglycoside 3adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.
- Molecular Weight
- expected: 30 kDa, apparent: 30 kDa
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