Insulin Autoantibody ELISA Kit
-
- Target
- Insulin Autoantibody
-
Reactivity
- Human, Pig, Cow
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
- Characteristics
-
ELISA kit for the detection of Insulin in the research laboratory
Alternative Names: Insulin ELISA kit
-
Insulin Autoantibody ELISA Kit
Reactivity: Human Colorimetric Competition ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Insulin Autoantibody ELISA KitReactivity: Human Colorimetric Indirect ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Insulin Autoantibody CLIA KitReactivity: Mouse Chemiluminescent Sandwich ELISA 31.25 pg/mL - 2000 pg/mL
-
- Application Notes
- Optimal conditions to be determined by end-user
- Plate
- Pre-coated
- Assay Procedure
-
AntiInsulin is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG class autoantibodies directed against insulin. The assay is based on microplates coated with a mixture of highly purified preparations of bovine, porcine and recombinant human insulin. The assay is useful in the primary diagnosis of Type I Diabetes, as well as for screening purposes to detect developing insulin autoantibodies in patients under insulin therapy. The microplate can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. During this procedure the binding of present autoantibodies, as well as the formation of the sandwich complexes and enzymatic colour reaction take place during three different reactionphases. To read the optical density a microplate reader with a 450 nm filter is required. Bichromatic measurement with a 600690 nm reference is recommended. The optical density for each calibrator may be graphically plotted against the concentration of IgG and unknowns extrapolated from the curve.
- Restrictions
- For Research Use only
-
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
-
- Target
- Insulin Autoantibody
- Target Type
- Antibody
- Background
-
Type I Diabetes is mainly characterised by limited or fully missing secretion of the hormone insulin. Morphological studies demonstrated a destruction of the beta cells of the so-called Langerhanns's che Cells (Islet Cells) in Type I diabetics. Numerous researchers described the appearance of antibodies directed against the islet cells and insulin as the causal reason for the onset of the disease. AntiInsulin antibodies are found in 37 percent of patients with newly detected Type I Diabetes, in 4 percent of their relatives of the first degree and in up to 1.5 percent of healthy controls. A positive correlation between the appearance of anti-Insulin and anti-Islet Cell antibodies has been reported. Anti-Insulin autoantibodies may be detected several months and in some cases years before the onset of the fully clinical manifestation of the diseases. Occasionally also autoantibodies to ProInsulin may appear.
Synonyms: Insulin ELISA kit.
-
