CCL3 ELISA Kit
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- Target See all CCL3 ELISA Kits
- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 23.44-1500 pg/mL
- Minimum Detection Limit
- 23.44 pg/mL
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of Human MIP-1Alpha /CCL3 concentrations in serum, plasma and other biological fluids.
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensitivity
- < 14 pg/mL
- Components
- plate, standard, diluent, antibodies
- Material not included
- pipettes, tubes, reader, incubator
- Featured
- Discover our best selling CCL3 ELISA Kit
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- Discover our top product CCL3 ELISA Kit
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- Sample Volume
- 100 μL
- Assay Time
- 3 - 4 h
- Plate
- Pre-coated
- Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human MIP-1Alpha /CCL3 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human MIP-1Alpha /CCL3 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MIP-1Alpha /CCL3 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human MIP-1Alpha /CCL3 can be calculated using the standard curve.
- Restrictions
- For Research Use only
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- Precaution of Use
- protect your eyes
- Storage
- 4 °C
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- Target See all CCL3 ELISA Kits
- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
- Alternative Name
- MIP-1alpha/CCL3 (Macrophage Inflammatory Protein-1alpha) (CCL3 Products)
- Synonyms
- MIP-1alpha ELISA Kit, G0S19-1 ELISA Kit, LD78ALPHA ELISA Kit, MIP-1-alpha ELISA Kit, MIP1A ELISA Kit, SCYA3 ELISA Kit, MIP-1a ELISA Kit, Scya3 ELISA Kit, AI323804 ELISA Kit, LD78alpha ELISA Kit, MIP1-(a) ELISA Kit, MIP1-alpha ELISA Kit, Mip1a ELISA Kit, CCL3L1 ELISA Kit, CCL3L3 ELISA Kit, chemokine (C-C motif) ligand 3 ELISA Kit, C-C motif chemokine ligand 3 ELISA Kit, CCL3 ELISA Kit, Ccl3 ELISA Kit
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Autophagy
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