TSG101 antibody (AA 229-319)

Catalog No. ABIN968913

Product Details anti-TSG101 Antibody

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Binding Specificity: AA 229-319

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This TSG101 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TSG101 aa. 229-319

Clone: 51-TSG101

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968586, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-TSG101 antibody (ABIN968913)

Li, Liao, Ruland, Mak, Cohen: "A TSG101/MDM2 regulatory loop modulates MDM2 degradation and MDM2/p53 feedback control." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 4, pp. 1619-24, (2001) (PubMed).

Zhong, Chen, Chen, Chen, Lee: "Identification of cellular TSG101 protein in multiple human breast cancer cell lines." in: Cancer research, Vol. 57, Issue 19, pp. 4225-8, (1997) (PubMed).

Li, Cohen: "Tsg101: a novel tumor susceptibility gene isolated by controlled homozygous functional knockout of allelic loci in mammalian cells." in: Cell, Vol. 85, Issue 3, pp. 319-29, (1996) (PubMed).

Target Details for TSG101

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Alternative Name: TSG101 (TSG101 Products)

Background: Tumor Susceptibility Gene 101 (TSG101) was identified in a random mutagenesis screen for potential tumor suppressors in NIH 3T3 cells. Altered transcripts of this gene have been detected in sporadic breast cancers and many other human malignancies. Though the function of TSG101 is not clearly understood, its protein structure includes motifs involved in transcription regulation, and TSG101 has been shown to modulate the activation of steroid hormone receptors. In addition, TSG101 may have a role in regulating ubiquination. The N-terminal region of TSG101 contains a domain that resembles the catalytically active region of ubiquitin conjugases. However, TSG101 lacks an active-site cysteine crucial to the function of these conjugases. Interestingly, TSG101 interferes with MDM2 ubiquination leading to a decrease in MDM2 decay and down-regulation of p53 protein. Thus, TSG101 may function as a dominant-negative inhibitor of ubiquination in pathways where protein expression is tightly regulated.
Synonyms: Tumor Susceptibility Gene 101

Molecular Weight: 46 kDa