PKA RIIalpha (AA 1-404) antibody

Catalog No. ABIN968783

Product Details

Target: PKA RIIalpha

Binding Specificity: AA 1-404

Reactivity: Human, Dog, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Cross-Reactivity: Dog (Canine), Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PKA[RIIalpha] aa. 1-404

Clone: 40-PKA[RIIalpha]

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968586

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References

Tanji, Yamamoto, Yorioka, Kohno, Kikuchi, Kikuchi: "A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 36955-61, (2002) (PubMed).

Fukuyama, Sueoka, Sugio, Otsuka, Niho, Akagi, Kozu: "MTG8 proto-oncoprotein interacts with the regulatory subunit of type II cyclic AMP-dependent protein kinase in lymphocytes." in: Oncogene, Vol. 20, Issue 43, pp. 6225-32, (2001) (PubMed).

Takahashi, Mukai, Oishi, Isagawa, Ono: "Association of immature hypophosphorylated protein kinase cepsilon with an anchoring protein CG-NAP." in: The Journal of biological chemistry, Vol. 275, Issue 44, pp. 34592-6, (2000) (PubMed).

Westphal, Soderling, Alto, Langeberg, Scott: "Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffold." in: The EMBO journal, Vol. 19, Issue 17, pp. 4589-600, (2000) (PubMed).

Sandberg, Skålhegg, Jahnsen: "The two mRNA forms for the type I alpha regulatory subunit of cAMP-dependent protein kinase from human testis are due to the use of different polyadenylation site signals." in: Biochemical and biophysical research communications, Vol. 167, Issue 1, pp. 323-30, (1990) (PubMed).

Target Details

Target: PKA RIIalpha

Background: CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic(C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIß, RIIalpha, and RIIß.These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cß, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Ralpha isoforms are ubiquitously expressed, the Rß isoforms are predominant in nervous and adipose tissues. Expression of the RIalpha subunit is modulated during muscle and adipocyte differentiation in vitro.

Molecular Weight: 51 kDa