VCP antibody (AA 9-130)

Catalog No. ABIN968778

Product Details anti-VCP Antibody

Target: VCP (Valosin Containing Protein (VCP))

Binding Specificity: AA 9-130

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This VCP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Human, Rat (Rattus), Dog (Canine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse VCP aa. 9-130

Clone: 18-VCP

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968540, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-VCP antibody (ABIN968778)

Zhang, Wang, Kajino, Greene: "VCP, a weak ATPase involved in multiple cellular events, interacts physically with BRCA1 in the nucleus of living cells." in: DNA and cell biology, Vol. 19, Issue 5, pp. 253-63, (2000) (PubMed).

Zhang, Liu, Kobayashi, Tonks: "Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17806-12, (1999) (PubMed).

Zhang, Ashendel, Becker, Morré: "Isolation and characterization of the principal ATPase associated with transitional endoplasmic reticulum of rat liver." in: The Journal of cell biology, Vol. 127, Issue 6 Pt 2, pp. 1871-83, (1995) (PubMed).

Egerton, Ashe, Chen, Druker, Burgess, Samelson: "VCP, the mammalian homolog of cdc48, is tyrosine phosphorylated in response to T cell antigen receptor activation." in: The EMBO journal, Vol. 11, Issue 10, pp. 3533-40, (1992) (PubMed).

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: VCP (VCP Products)

Background: ATPases that belong to the ATPase associated with different cellular activities (AAA) family are homo-oligomeric proteins that have roles in cell cycle regulation, protein degradation, organelle biogenesis, and vesicle mediated-protein transport. Valosin containing protein (VCP) is a AAA family member that is found as a hexamer in rat liver. In vitro, VCP catalyzes the hydrolysis of ATP, but this activity is distinct from classical transport ATPases. VCP protein is found primarily in the transitional elements between the rough and smooth ER (TER), but stimulation with EGF leads to VCP translocation to the nucleus. Antibodies to VCP can perturb cell-free formation of transition vesicles from isolated TER of rat liver, and can inhibit transfer of material from ER to Golgi in a reconstituted membrane system. VCP is phosphorylated after T-cell activation, and PTPH1 dephosphorylation of VCP correlates with suppression of cell growth. In addition, the N-terminal region of VCP binds to the DNA damage repair protein, BRCA1. Thus, VCP may have roles in both ER protein transport and nuclear function, which are important for cell growth and survival. This antibody is routinely tested by western blot analysis.
Synonyms: Valosin Containing Protein

Molecular Weight: 97 kDa

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway