DARPP32 antibody (AA 70-181)

Catalog No. ABIN968572

Product Details anti-DARPP32 Antibody

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Binding Specificity: AA 70-181

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This DARPP32 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human DARPP-32 aa. 70-181

Clone: 15-DARPP

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-DARPP32 antibody (ABIN968572)

Bibb, Snyder, Nishi, Yan, Meijer, Fienberg, Tsai, Kwon, Girault, Czernik, Huganir, Hemmings, Nairn, Greengard: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, (2000) (PubMed).

Yan, Feng, Fienberg, Greengard: "D(2) dopamine receptors induce mitogen-activated protein kinase and cAMP response element-binding protein phosphorylation in neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11607-12, (1999) (PubMed).

Yan, Hsieh-Wilson, Feng, Tomizawa, Allen, Fienberg, Nairn, Greengard: "Protein phosphatase 1 modulation of neostriatal AMPA channels: regulation by DARPP-32 and spinophilin." in: Nature neuroscience, Vol. 2, Issue 1, pp. 13-7, (1999) (PubMed).

Fienberg, Hiroi, Mermelstein, Song, Snyder, Nishi, Cheramy, OCallaghan, Miller, Cole, Corbett, Haile, Cooper, Onn, Grace, Ouimet, White, Hyman, Surmeier, Girault, Nestler, Greengard: "DARPP-32: regulator of the efficacy of dopaminergic neurotransmission." in: Science (New York, N.Y.), Vol. 281, Issue 5378, pp. 838-42, (1998) (PubMed).

Kurihara, Lewis, Eisler, Greengard: "Cloning of cDNA for DARPP-32, a dopamine- and cyclic AMP-regulated neuronal phosphoprotein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 2, pp. 508-17, (1988) (PubMed).

Target Details for DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Alternative Name: DARPP-32 (PPP1R1B Products)

Background: Dopaminergic signaling in midbrain neurons is essential to multiple brain functions and involves the activation of dopamine receptors,nsuch as D1 and D2, which regulate the phosphorylation state of DARPP-32 (dopamine and cyclic AMP-regulated phospho-protein of Mr = 32,000). D1 receptor ligation causes activation of PKA and phosphorylation of DARPP-32 at Thr-34, which converts DARPP-32 to a potent inhibitor of protein phosphatase 1 (PP1). In addition, DARPP-32 is converted to an inhibitor of PKA via phosphorylation at Thr-75 by cyclin-dependent kinase 5 (Cdk5). D2 receptor ligation inhibits PKA and activates protein phosphatase 2B/calcineurin causing dephosphorylation of DARPP-32. The major function of DARPP-32 may be to inhibit the activity of PP1, which controls the phosphorylation state of neurotransmitter receptors, ion channels, ion pumps, and transcription factors. DARPP-32 -/- mice are defective in the physiologicial and behavioral responses to dopamine. Thus, DARPP-32, a bifunctional signal transduction molecule that differentially controls a Ser/Thr kinase and a Ser/Thr phosphatase, is a critical element of dopaminergic neurotransmission and normal brain function.

Molecular Weight: 32 kDa