GIT1 antibody (AA 664-770)

Catalog No. ABIN968516

Product Details anti-GIT1 Antibody

Target: GIT1

Binding Specificity: AA 664-770

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GIT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat GIT1 aa. 664-770

Clone: 13-GIT1

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-GIT1 antibody (ABIN968516)

Zhao, Manser, Loo, Lim: "Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly." in: Molecular and cellular biology, Vol. 20, Issue 17, pp. 6354-63, (2000) (PubMed).

Premont, Claing, Vitale, Freeman, Pitcher, Patton, Moss, Vaughan, Lefkowitz: "beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 24, pp. 14082-7, (1998) (PubMed).

Target Details for GIT1

Target: GIT1

Alternative Name: GIT1 (GIT1 Products)

Background: Activation of adrenergic receptors (AR), such as beta2AR leads to activation of heterotrimeric G proteins and recruitment of G protein-coupled receptor kinases (GRKs). Phosphorylation of the activated receptor by GRKs promotes the binding of arrestin protein which prevents further G protein activation. GRK interactor 1 (GIT1) was identified in a yeast two-hybrid screen for proteins that bind GRK2. GIT1 has an N-terminal Zn2+ finger-like motif involved in GTPase activating activity and multipe ankyrin repeat units. In vitro, GIT1 is a GTPase-activating protein (GAP) for the ADP ribosylation factor (ARF) family of small GTP-binding proteins. In rat, GIT1 is widely expressed with the highest levels in the testis and lowest levels in the liver and spleen. Overexpression of GIT1 in HEK293 cells causes reduced beta2AR signaling and increased receptor phosphorylation as a result of reduced receptor internalization and resensitization. In addition, GIT1 interacts directly with paxillin and the PIX exchange factors, and indirectly with PAKs, Rac1, and Nck. GIT proteins are also now known to be PIP3-stimulated GAPs for ARF6. Thus, GIT1 activation of ARF proteins may involve cell signaling pathways that are important for endocytosis, cell adhesion, and cytoskeletal dynamics.
Synonyms: G protein-coupled receptor kinase interactor 1

Molecular Weight: 95 kDa