NMT2 antibody (AA 10-119)

Catalog No. ABIN968466

This anti-NMT2 antibody is a Mouse Monoclonal antibody detecting NMT2 in WB and IF. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for NMT2 antibody (AA 10-119) (ABIN968466)

Target: NMT2 (N-Myristoyltransferase 2 (NMT2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NMT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 30-NMT

Product Details anti-NMT2 Antibody

Binding Specificity: AA 10-119

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human NMT-2 aa. 10-119

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-NMT2 antibody (ABIN968466)

Giang, Cravatt: "A second mammalian N-myristoyltransferase." in: The Journal of biological chemistry, Vol. 273, Issue 12, pp. 6595-8, (1998) (PubMed).

Target Details for NMT2

Target: NMT2 (N-Myristoyltransferase 2 (NMT2))

Alternative Name: NMT-2

Background: Myristoylation is an essential cotranslational modification for many mammalian, viral, and fungal signaling proteins. N-terminal myristoylation is a lipid modification that is catalyzed by N-myristoyltransferase (NMT). NMT transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. This modification is important for localization and/or function of many of these proteins. Two human NMTs (NMT-1, NMT-2) have been identified. These proteins share 95% amino acid identity with their mouse homologs. NMT-1 is processed to form four protein isoforms with molecular weights ranging from 49-68 kDa. It is not known if these four isoforms are derived from single or multiple gene(s) and their exact functional roles are yet to be determined. In contrast to NMT-1, NMT-2 is a single 65 kDa protein. It contains 77% sequence identity with NMT-1, indicating that these proteins comprise two distinct families with overlapping, but distinct, substrate preferences. Additionally, NMT enzymatic activity is increased in colorectal tumors. Thus, NMTs are transferases whose function is essential for biological function of a variety of signaling proteins.

Molecular Weight: 65 kDa

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling