RPS6KB1 antibody (AA 2-121)

Catalog No. ABIN968441

Product Details anti-RPS6KB1 Antibody

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Binding Specificity: AA 2-121

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RPS6KB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat p70 [S6K] aa. 2-121

Clone: 16-p70[s6k]

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968548, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-RPS6KB1 antibody (ABIN968441)

Brennan, Babbage, Thomas, Cantrell: "p70(s6k) integrates phosphatidylinositol 3-kinase and rapamycin-regulated signals for E2F regulation in T lymphocytes." in: Molecular and cellular biology, Vol. 19, Issue 7, pp. 4729-38, (1999) (PubMed).

Johanson, Naccache, Crouch: "A p85 subunit-independent p110alpha PI 3-kinase colocalizes with p70 S6 kinase on actin stress fibers and regulates thrombin-stimulated stress fiber formation in swiss 3T3 cells." in: Experimental cell research, Vol. 248, Issue 1, pp. 223-33, (1999) (PubMed).

Romanelli, Martin, Toker, Blenis: "p70 S6 kinase is regulated by protein kinase Czeta and participates in a phosphoinositide 3-kinase-regulated signalling complex." in: Molecular and cellular biology, Vol. 19, Issue 4, pp. 2921-8, (1999) (PubMed).

Ming, Burgering, Wennström, Claesson-Welsh, Heldin, Bos, Kozma, Thomas: "Activation of p70/p85 S6 kinase by a pathway independent of p21ras." in: Nature, Vol. 371, Issue 6496, pp. 426-9, (1994) (PubMed).

Target Details for RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Alternative Name: p70 s6k (RPS6KB1 Products)

Background: Increased protein synthesis, an obligatory step in cell proliferation, is regulated by the phosphorylation of a number of translational components, such as ribosomal protein S6. p70 S6 Kinase (p70 [S6K]), a cytosolic kinase that is primarily responsible for S6 phosphorylation, belongs to a family of at least six S6 kinases. p70 [S6K] and p85 [S6K] are two isoforms of the same kinase. Although they are encoded by the same gene, p80 [S6K] contains a 23 amino acid N-terminal extension that serves as a nuclear localization sequence. These kinases are located in a p21 ras-independent signaling pathway and are regulated by Ser/Thr phosphorylation in response to mitogenic stimulation. Activation of p70 [S6K] requires sequential phosphorylations within the autoinhibitory domain and at Thr 389. p70 [S6K] associates with PDK-1 and PKCzeta to form a possible PI3-K signaling complex. The immunosuppressive agent rapamycin forms an intracellular complex with FKBP12 and FRAP (RAFT1) that inhibits phosphorylation of p70 [S6K] at Thr 389 and, in turn, prevents enzyme activation. Thus, p70 [S6K] is an important regulator of cell proliferation and a potential target of agents that modify the immune and proliferative responses. p70 [S6K] has been reported to be observable migrating within a range of 60-70 kD by western blotting.
Synonyms: p70 S6 Kinase

Molecular Weight: 60-70 kDa

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect