Aurora Kinase B antibody (AA 2-124)

Catalog No. ABIN968358

Product Details anti-Aurora Kinase B Antibody

Target: Aurora Kinase B (AURKB)

Binding Specificity: AA 2-124

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat AIM-1 aa. 2-124

Clone: 6-AIM

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968537

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Aurora Kinase B antibody (ABIN968358)

Trinkle-Mulcahy, Andrews, Wickramasinghe, Sleeman, Prescott, Lam, Lyon, Swedlow, Lamond: "Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 107-17, (2003) (PubMed).

Chen, Jin, Tahir, Zhang, Liu, Sarthy, McGonigal, Liu, Rosenberg, Ng: "Survivin enhances Aurora-B kinase activity and localizes Aurora-B in human cells." in: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 486-90, (2002) (PubMed).

Lange, Rebollo, Herold, González: "Cdc37 is essential for chromosome segregation and cytokinesis in higher eukaryotes." in: The EMBO journal, Vol. 21, Issue 20, pp. 5364-74, (2002) (PubMed).

Tatsuka, Katayama, Ota, Tanaka, Odashima, Suzuki, Terada: "Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells." in: Cancer research, Vol. 58, Issue 21, pp. 4811-6, (1998) (PubMed).

Terada, Tatsuka, Suzuki, Yasuda, Fujita, Otsu: "AIM-1: a mammalian midbody-associated protein required for cytokinesis." in: The EMBO journal, Vol. 17, Issue 3, pp. 667-76, (1998) (PubMed).

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: AIM-1 (AURKB Products)

Synonyms: AIK2 antibody, AIM-1 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, IPL1 antibody, PPP1R48 antibody, STK12 antibody, STK5 antibody, aurkb-sv1 antibody, aurkb-sv2 antibody, AIRK2 antibody, AL022959 antibody, Aik2 antibody, Aim1 antibody, Ark2 antibody, STK-1 antibody, Stk12 antibody, Stk5 antibody, Aur B antibody, Aurora B antibody, Aurora-B antibody, CG6620 antibody, DmAIRK2 antibody, DmAurB antibody, DmAuroraB antibody, Dmel\\CG6620 antibody, IAL antibody, IAL1 antibody, Ial antibody, aurB antibody, aurora B antibody, ARK-2 antibody, XAIRK2 antibody, aik2 antibody, aim-1 antibody, aim1 antibody, airk2 antibody, ark2 antibody, ipl1 antibody, stk12 antibody, stk5 antibody, CHUNP6896 antibody, fa09g06 antibody, stka antibody, wu:fa09g06 antibody, aurkb antibody, AIRK2-B antibody, XAIRK2-B antibody, aurkb-a antibody, aurkb-b antibody, xAurora-B-B antibody, aurora kinase B antibody, aurora B antibody, aurora kinase B S homeolog antibody, AURKB antibody, Aurkb antibody, aurB antibody, aurkb antibody, aurkb.S antibody

Background: The mitotic phase of the cell cycle is a complex process that ensures the fidelity of chromosome segregation. During the final stage of mitosis (telophase), segregated chromosomes become less condense and nuclear membranes surround the two sets of daughter chromosomes. Simultaneously, the separation and segregation of the cytoplasm (cytokinesis) ensures complete division and formation of two identical daughter cells. Regulation of cytokinesis is poorly understood and errors in this process can lead to cell death or oncogenesis. The Drosophila serine/threonine protein kinase Aurora and the S. cerevisiae Ipl1 kinase are highly homologous and are required for progression through mitosis. Their mammalian homolog AIM-1 (also known as Aurora and Ipl1-like midbody associated protein) accumulates at the G2/M interface. During late anaphase, AIM-1 is found at the equator of central spindles. However, during telophase and cytokinesis, it is found at the midbody. Although over-expression of a kinase-inactive AIM-1 mutant disrupts formation of the cleavage furrow, nuclear division is unaffected. Thus, it is thought that AIM-1 is essential for cleavage furrowing and the onset of cytokinesis.
Synonyms: Aurora B, Aurora and Ipl1-like midbody associated protein

Molecular Weight: 41 kDa

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades