RABEP1 antibody (AA 247-417)

Catalog No. ABIN968104

Product Details anti-RABEP1 Antibody

Target: RABEP1 (Rabaptin, RAB GTPase Binding Effector Protein 1 (RABEP1))

Binding Specificity: AA 247-417

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RABEP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Rabaptin-5 aa. 247-417.

Clone: 20-Rabaptin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968585, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-RABEP1 antibody (ABIN968104)

Shiba, Takatsu, Shin, Nakayama: "Gamma-adaptin interacts directly with Rabaptin-5 through its ear domain." in: Journal of biochemistry, Vol. 131, Issue 3, pp. 327-36, (2002) (PubMed).

Zhu, Doray, Poussu, Lehto, Kornfeld: "Binding of GGA2 to the lysosomal enzyme sorting motif of the mannose 6-phosphate receptor." in: Science (New York, N.Y.), Vol. 292, Issue 5522, pp. 1716-8, (2001) (PubMed).

Stenmark, Vitale, Ullrich, Zerial: "Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion." in: Cell, Vol. 83, Issue 3, pp. 423-32, (1996) (PubMed).

Target Details for RABEP1

Target: RABEP1 (Rabaptin, RAB GTPase Binding Effector Protein 1 (RABEP1))

Alternative Name: Rabaptin-5 (RABEP1 Products)

Synonyms: RAB5EP antibody, RABPT5 antibody, AA409789 antibody, AI481320 antibody, AV213537 antibody, AW240603 antibody, Rab5ep antibody, rabaptin-5 antibody, rabaptin, RAB GTPase binding effector protein 1 antibody, RABEP1 antibody, Rabep1 antibody

Background: Rabs are small GTP-binding proteins that are localized to specific intracellular vesicles and organelles. They are important for the regulation of vesicular traffic. It is thought that Rabs cycle between GTP- and GDP-bound forms and this cycle is related to their function. Rabaptin-5 is protein that interacts with GTP-Rab5. Although Rabaptin-5 is localized in the cytosol, it is also recruited to the endosomal fraction in a Rab5 and GTP-dependent manner. The removal of Rabaptin-5 from the cytosol substantially impairs GTP and Rab5-dependent endosomal fusion. This indicates that Rabaptin-5 may be an important partner of Rab5 during endocytosis. Rabaptin-5 contains 862 amino acids and both ends of the protein contain coiled-coil domains typically found in vesicular transport proteins. This antibody is routinely tested by western blot analysis.

Molecular Weight: 117 kDa