PP2A Catalytic alpha (AA 153-309) antibody

Catalog No. ABIN968035

Product Details

Target: PP2A Catalytic alpha

Binding Specificity: AA 153-309

Reactivity: Dog, Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PP2A aa. 153-309

Clone: 46

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References

Efendiev, Yudowski, Zwiller, Leibiger, Katz, Berggren, Pedemonte, Leibiger, Bertorello: "Relevance of dopamine signals anchoring dynamin-2 to the plasma membrane during Na+,K+-ATPase endocytosis." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44108-14, (2002) (PubMed).

Nunbhakdi-Craig, Machleidt, Ogris, Bellotto, White, Sontag: "Protein phosphatase 2A associates with and regulates atypical PKC and the epithelial tight junction complex." in: The Journal of cell biology, Vol. 158, Issue 5, pp. 967-78, (2002) (PubMed).

Reiken, Gaburjakova, Guatimosim, Gomez, DArmiento, Burkhoff, Wang, Vassort, Lederer, Marks: "Protein kinase A phosphorylation of the cardiac calcium release channel (ryanodine receptor) in normal and failing hearts. Role of phosphatases and response to isoproterenol." in: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 444-53, (2002) (PubMed).

Yang, Fan, Wadzinski, Sakurai, Richmond: "Protein phosphatase 2A interacts with and directly dephosphorylates RelA." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 47828-33, (2001) (PubMed).

Chen, Parsons, Brautigan: "Tyrosine phosphorylation of protein phosphatase 2A in response to growth stimulation and v-src transformation of fibroblasts." in: The Journal of biological chemistry, Vol. 269, Issue 11, pp. 7957-62, (1994) (PubMed).

Target Details

Target: PP2A Catalytic alpha

Background: Protein phosphatases of the type 2A (PP2A) are composed of two regulatory and one catalytic subunit. The different subunits are encoded by multiple genes. PP2A are specific for phosphoserine and phosphothreonine residues of proteins that are involved in the regulation of cellular growth. PP2A binds and dephosphorylates the middle T antigens of polyoma virus. This interaction may regulate the transcriptional activity of the virus. The 36 kDa catalytic subunit is rapidly tyrosine phosphorylated as a result of growth factor stimulation or cell transformation. Tyrosine phosphorylation of the catalytic subunit may inactivate the phosphatase activity of PP2A.

Molecular Weight: 36 kDa