CaM Kinase Kinase (AA 341-504) antibody

Catalog No. ABIN968028

Product Details

Target: CaM Kinase Kinase

Binding Specificity: AA 341-504

Reactivity: Dog, Human, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Cross-Reactivity: Human, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat CaM Kinase Kinase aa. 341-504

Clone: 6-CaM Kinase Kinase

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References

Egea, Espinet, Soler, Dolcet, Yuste, Encinas, Iglesias, Rocamora, Comella: "Neuronal survival induced by neurotrophins requires calmodulin." in: The Journal of cell biology, Vol. 154, Issue 3, pp. 585-97, (2001) (PubMed).

Wayman, Walters, Kolibaba, Soderling, Christian: "CaM kinase IV regulates lineage commitment and survival of erythroid progenitors in a non-cell-autonomous manner." in: The Journal of cell biology, Vol. 151, Issue 4, pp. 811-24, (2000) (PubMed).

Wu, Means: "Ca(2+)/calmodulin-dependent protein kinase IV is expressed in spermatids and targeted to chromatin and the nuclear matrix." in: The Journal of biological chemistry, Vol. 275, Issue 11, pp. 7994-9, (2000) (PubMed).

Edelman, Mitchelhill, Selbert, Anderson, Hook, Stapleton, Goldstein, Means, Kemp: "Multiple Ca(2+)-calmodulin-dependent protein kinase kinases from rat brain. Purification, regulation by Ca(2+)-calmodulin, and partial amino acid sequence." in: The Journal of biological chemistry, Vol. 271, Issue 18, pp. 10806-10, (1996) (PubMed).

Tokumitsu, Enslen, Soderling: "Characterization of a Ca2+/calmodulin-dependent protein kinase cascade. Molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase." in: The Journal of biological chemistry, Vol. 270, Issue 33, pp. 19320-4, (1995) (PubMed).

Target Details

Target: CaM Kinase Kinase

Background: Ca2+/Calmodulin (CaM)-dependent kinases are multifunctional kinases involved in a myriad of cellular functions such as neurotransmitter synthesis, long-term potentiation, and formation of spatial learning. A 68kDa CaM Kinase Kinase with homology to the Ca2+/CaM-dependent kinases, phosphorylates and activates Ca2+/CaM-dependent protein kinases I and IV, but not type II. This phosphorylation occurs on Ser/Thr residues and is Ca2+ and CaM dependent. CaM Kinase Kinase is abundant in brain tissue, and is thought to be an upstream regulator in the Ca2+/CaM-dependent neural processes. This antibody is routinely tested by western blot analysis.

Molecular Weight: 68 kDa