eIF-4gamma (AA 1217-1386) antibody

Catalog No. ABIN968023

Product Details

Target: eIF-4gamma

Binding Specificity: AA 1217-1386

Reactivity: Dog, Human

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Cross-Reactivity: Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human eIF-4gamma aa. 1217-1386

Clone: 40-eIF

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References

Kedersha, Chen, Gilks, Li, Miller, Stahl, Anderson: "Evidence that ternary complex (eIF2-GTP-tRNA(i)(Met))-deficient preinitiation complexes are core constituents of mammalian stress granules." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 195-210, (2002) (PubMed).

Gan, Rhoads: "Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation." in: The Journal of biological chemistry, Vol. 271, Issue 2, pp. 623-6, (1996) (PubMed).

Yan, Rychlik, Etchison, Rhoads: "Amino acid sequence of the human protein synthesis initiation factor eIF-4 gamma." in: The Journal of biological chemistry, Vol. 267, Issue 32, pp. 23226-31, (1992) (PubMed).

Target Details

Target: eIF-4gamma

Background: EIF-4 proteins are required for mRNA recognition and acceleration of translation during protein synthesis. This group of proteins consists of the RNA helicase, eIF-4A, the RNA-binding protein, eIF-4B, and the cap binding proteins, eIF-4E and eIF-4gamma. eIF-4gamma, also referred to as p220, is a 1396 amino acid polypeptide that migrates as a 220kDa. eIF-4gamma has several potential glycosylation and phosphorylation sites that could account for its anomalous electrophoretic mobility. In addition, eIF-4gamma is rapidly degraded in picornavirus-infected cells due to its content of multiple PEST sequences.

Molecular Weight: 220 kDa