STAT2 antibody (AA 1-178)

Catalog No. ABIN967806

Product Details anti-STAT2 Antibody

Target: STAT2 (Signal Transducer and Activator of Transcription 2, 113kDa (STAT2))

Binding Specificity: AA 1-178

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Stat2 aa. 1-178

Clone: 22-Stat2

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968586, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-STAT2 antibody (ABIN967806)

Blesofsky, Mowen, Arduini, Baker, Murphy, Bowtell, David: "Regulation of STAT protein synthesis by c-Cbl." in: Oncogene, Vol. 20, Issue 50, pp. 7326-33, (2001) (PubMed).

Russell-Harde, Wagner, Rani, Vogel, Colamonici, Ransohoff, Majchrzak, Fish, Perez, Croze: "Role of the intracellular domain of the human type I interferon receptor 2 chain (IFNAR2c) in interferon signaling. Expression of IFNAR2c truncation mutants in U5A cells." in: The Journal of biological chemistry, Vol. 275, Issue 31, pp. 23981-5, (2000) (PubMed).

Duff, Quinlan, Paxton, Naik, Caughman: "Pervanadate mimics IFNgamma-mediated induction of ICAM-1 expression via activation of STAT proteins." in: The Journal of investigative dermatology, Vol. 108, Issue 3, pp. 295-301, (1997) (PubMed).

Ruff-Jamison, Chen, Cohen: "Induction by EGF and interferon-gamma of tyrosine phosphorylated DNA binding proteins in mouse liver nuclei." in: Science (New York, N.Y.), Vol. 261, Issue 5129, pp. 1733-6, (1993) (PubMed).

Fu, Schindler, Improta, Aebersold, Darnell: "The proteins of ISGF-3, the interferon alpha-induced transcriptional activator, define a gene family involved in signal transduction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7840-3, (1992) (PubMed).

Target Details for STAT2

Target: STAT2 (Signal Transducer and Activator of Transcription 2, 113kDa (STAT2))

Alternative Name: Stat2 (STAT2 Products)

Background: The Stat proteins function as both cytoplasmic signal transducers and activators of transcription. Stat91/84 (the two proteins are the result of alternate splicing-Stat91 has an additional 38 C-terminal amino acids) and Stat113 were the first identified members of this protein family. These three polypeptides contain both SH2 and SH3 domains and have also been described as members of the ISGF3 (interferon-stimulated gene factor 3) complex. With the discovery of additional members of the Stat family (Stats3 & 4), the nomenclature has been revised to indicate the Stat family members in the order of their discovery. Stat 91, 84, and 113 have become Stat1alpha, Stat1beta, and Stat2, respectively. In response to IFN-alpha treatment, Stat1alpha, Stat1beta, and Stat2 become tyrosine-phosphorylated and migrate to the nucleus where they join a 48 kDa DNA binding protein and subsequently direct the transcription at IFN-alpha responsive elements. The genes encoding Stat2 and Stat1 are closely related. An extended acidic C-terminal region exclusive to Stat2 implicates a potential role in gene activation. Stat2 phosphorylation can occur independently of Stat1alpha, but phosphorylated Stat2 does appear to be dependent on its interaction with Stat1alpha for efficient nuclear localization and/or stability.

Molecular Weight: 113 kDa

Pathways: JAK-STAT Signaling, Hepatitis C, CXCR4-mediated Signaling Events