N-Cadherin antibody (Extracellular Domain)

Catalog No. ABIN967678

Product Details anti-N-Cadherin Antibody

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), BioImaging (BI)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Please refer to us for technical protocols.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human extracellular N-Cadherin domain Recombinant Protein

Clone: 8C11

Isotype: IgG1 kappa

Application Details

Application Notes: Because the extracellular domain of N-Cadherin is trypsin-sensitive, it is important to avoid using trypsin to dissociate the cells to be studied.

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-N-Cadherin antibody (ABIN967678)

Wein, Pietsch, Saffrich, Wuchter, Walenda, Bork, Horn, Diehlmann, Eckstein, Ho, Wagner: "N-cadherin is expressed on human hematopoietic progenitor cells and mediates interaction with human mesenchymal stromal cells." in: Stem cell research, Vol. 4, Issue 2, pp. 129-39, (2010) (PubMed).

Puch, Armeanu, Kibler, Johnson, Müller, Wheelock, Klein: "N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation." in: Journal of cell science, Vol. 114, Issue Pt 8, pp. 1567-77, (2001) (PubMed).

Knudsen, Soler, Johnson, Wheelock: "Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin." in: The Journal of cell biology, Vol. 130, Issue 1, pp. 67-77, (1995) (PubMed).

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CD325 (CDH2 Products)

Background: The 8C11 monoclonal antibody recognizes the extracellular domain of human N-Cadherin (CD325). Cadherins are a family of Ca2+ dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenins (alpha, beta, gamma). Members of the cadherin family include P-cadherin , E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-cadherin, cadherin 5, L-CAM, and EP-cadherin. N-cadherin mRNA is found at elevated levels in brain and heart and at a much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation, and protease-mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogenous kinases and phosphatases. Tyrosine phosphorylation of beta-catenin complexed with N-cadherin results in dissociation of N-cadherin from actin. However, N-cadherin also interacts with a PTP1B-like phosphatase that dephosphorylates beta-catenin and promotes N-cadherin/actin association. Thus, N-cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events.
Synonyms: Cadherin-2, N-Cadherin

Molecular Weight: 130 kDa

Gene ID: 1000

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane