SOX2 antibody

Catalog No. ABIN967676

Product Details anti-SOX2 Antibody

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Intracellular Staining (ICS), BioImaging (BI)

Brand: BD Pharmingen™

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. mTESR™1 is a trademark of StemCell Technologies.
5. Triton is a trademark of the Dow Chemical Company.
6. Alexa Fluor® is a registered trademark of Life Technologies Corporation.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Sox2 Recombinant Protein

Clone: O30-678

Isotype: IgG1 kappa

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-SOX2 antibody (ABIN967676)

Pan, Thomson: "Nanog and transcriptional networks in embryonic stem cell pluripotency." in: Cell research, Vol. 17, Issue 1, pp. 42-9, (2007) (PubMed).

Takahashi, Yamanaka: "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors." in: Cell, Vol. 126, Issue 4, pp. 663-76, (2006) (PubMed).

Boyer, Lee, Cole, Johnstone, Levine, Zucker, Guenther, Kumar, Murray, Jenner, Gifford, Melton, Jaenisch, Young: "Core transcriptional regulatory circuitry in human embryonic stem cells." in: Cell, Vol. 122, Issue 6, pp. 947-56, (2005) (PubMed).

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2 (SOX2 Products)

Background: The monoclonal antibody O30-678 recognizes the Sox2 transcription factor. Sox2 [SRY (sex determining region Y)-box 2] is a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox2 is required for the maintenance of the undifferentiated state of pluripotent stem cells. Complexes of Sox2 with the homeobox transcription factors Oct3/4 and/or Nanog bind to the promoters of a network of genes that are involved in the maintenance of pluripotency and self renewal in stem cells. Sox2 is also a marker of neural stem cells during embryonic development and in the adult brain. The O30-678 antibody recognizes both human and mouse Sox2 proteins.

Molecular Weight: 36 kDa

Gene ID: 6657, 20674

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis