ITGB1 antibody

Catalog No. ABIN967623

Product Details anti-ITGB1 Antibody

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: (C57BL/6 x DBA/2)F1 mouse bone-marrow stromal cell clone BMS2

Clone: KMI6

Isotype: IgG2a kappa

Application Details

Application Notes: Please note that the epitope is lost upon sulfhydryl reduction of the antigen. For IHC, we recommend the use of purified anti-mouse CD29 mAb 9EG7 mAb in our special formulation for immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-ITGB1 antibody (ABIN967623)

Wadsworth, Chang, Hong, Halvorson, Otto, Coligan: "Expression of a novel integrin beta 1 chain epitope and anti-beta 1 antibody-mediated enhancement of fibronectin binding are dependent on the stage of T cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 5, pp. 2125-33, (1995) (PubMed).

Wu, Miyake, Medina, Kincade, Gimble: "Recognition of murine integrin beta 1 by a rat anti-stromal cell monoclonal antibody." in: Hybridoma, Vol. 13, Issue 5, pp. 409-16, (1995) (PubMed).

Jacobsen, Miyake, Kincade, Osmond: "Highly restricted expression of a stromal cell determinant in mouse bone marrow in vivo." in: The Journal of experimental medicine, Vol. 176, Issue 4, pp. 927-35, (1992) (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, (1990) (PubMed).

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: CD29 (ITGB1 Products)

Background: The KMI6 antibody reacts with the 130-kDa integrin ß1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin achains. With a1 through a6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with av (CD51), it forms avß1 integrin. It also associates with the integrin a7, a8, and a9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including leukocytes, endothelia, smooth muscle, epithelia, and oocytes. The epitope recognized by the KMI6 mAb can be detected by immunoblot in unreduced extracts from a variety of cells and tissues having a number of different ß1-integrin heterodimer combinations. However, the epitope is not well expressed on the surface of the majority of hematopoietic cells. It is detected on the surface of fetal liver cells, some bone marrow stromal cells, and some cultured hematopoietic cell lines, including P815 and PA6. Surface expression of the CD29 epitope recognized by mAb KMI6 on thymocytes and T lymphocytes appears to be developmentally regulated, and KMI6 antibody enhances the adhesion of KMI6 thymocytes to fibronectin and laminin.
Synonyms: Integrin beta1 chain

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome