TEK antibody (Extracellular Domain)

Catalog No. ABIN967565

Product Details anti-TEK Antibody

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Binding Specificity: Extracellular Domain

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TEK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TIE2 extracellular domain Recombinant Protein

Clone: 33

Isotype: IgG1 kappa

Application Details

Comment:

Related Products: ABIN967389, ABIN968537

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-TEK antibody (ABIN967565)

Partanen, Dumont: "Functions of Tie1 and Tie2 receptor tyrosine kinases in vascular development." in: Current topics in microbiology and immunology, Vol. 237, Issue 4, pp. 159-72, (1999) (PubMed).

Peters, Coogan, Berry, Marks, Iglehart, Kontos, Rao, Sankar, Trogan: "Expression of Tie2/Tek in breast tumour vasculature provides a new marker for evaluation of tumour angiogenesis." in: British journal of cancer, Vol. 77, Issue 1, pp. 51-6, (1998) (PubMed).

Wong, Haroon, Werner, Dewhirst, Greenberg, Peters: "Tie2 expression and phosphorylation in angiogenic and quiescent adult tissues." in: Circulation research, Vol. 81, Issue 4, pp. 567-74, (1997) (PubMed).

Dumont, Gradwohl, Fong, Puri, Gertsenstein, Auerbach, Breitman: "Dominant-negative and targeted null mutations in the endothelial receptor tyrosine kinase, tek, reveal a critical role in vasculogenesis of the embryo." in: Genes & development, Vol. 8, Issue 16, pp. 1897-909, (1994) (PubMed).

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: TIE2 (TEK Products)

Background: The TIE receptor family is a member of the class of receptors called the receptor tyrosine kinases (RKT). RKTs are cell surface receptors which also contain a protein kinase domain in their intracellular portion and upon binding of their specific growth factor, initiate a series of downstream biological events. TIE receptors, designated Tie1 and Tie2 (Tek) have a tissue distribution which is limited primarily to cells of endothelial origin. Tie2 has been shown to be required for the normal development of vascular structures during embryogenesis. It has also been demonstrated to play a role in normal and pathological angiogenesis in adults. Tie2 has a predicted molecular weight of 126 kD, but may be observable at ~130-165 kDa. This antibody has been reported to recognize human and mouse Tie2 but not Tie1.

Molecular Weight: 130-165 kDa

Pathways: RTK Signaling, Growth Factor Binding