ASCL1 antibody

Catalog No. ABIN967560

Product Details anti-ASCL1 Antibody

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: Western Blotting (WB)

Brand: BD Pharmingen™

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat MASH1 full length recombinant protein

Clone: 24B72D11-1

Isotype: IgG1

Application Details

Application Notes: The 24B72D11.1 antibody is recommended for western blot analysis (0.5-2.0 µg/ml). Rat embryonic brain can be used as a positive control. Neuro2A mouse neuroblastoma cells (ATCC CCL-131) may also be used as a positive control for this application.

The 24B72D11.1 antibody is reported in the literature to work well for immunohistochemistry.

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ASCL1 antibody (ABIN967560)

Wang, Long, Flandin, Pla, Waclaw, Campbell, Rubenstein: "Loss of Gsx1 and Gsx2 function rescues distinct phenotypes in Dlx1/2 mutants." in: The Journal of comparative neurology, Vol. 521, Issue 7, pp. 1561-84, (2013) (PubMed).

Krolewski, Packard, Schwob: "Global expression profiling of globose basal cells and neurogenic progression within the olfactory epithelium." in: The Journal of comparative neurology, Vol. 521, Issue 4, pp. 833-59, (2013) (PubMed).

Sawamoto, Hirota, Alfaro-Cervello, Soriano-Navarro, He, Hayakawa-Yano, Yamada, Hikishima, Tabata, Iwanami, Nakajima, Toyama, Itoh, Alvarez-Buylla, Garcia-Verdugo, Okano: "Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain." in: The Journal of comparative neurology, Vol. 519, Issue 4, pp. 690-713, (2011) (PubMed).

Guo, Packard, Krolewski, Harris, Manglapus, Schwob: "Expression of pax6 and sox2 in adult olfactory epithelium." in: The Journal of comparative neurology, Vol. 518, Issue 21, pp. 4395-418, (2011) (PubMed).

Ivaniutsin, Chen, Mason, Price, Pratt: "Adenomatous polyposis coli is required for early events in the normal growth and differentiation of the developing cerebral cortex." in: Neural development, Vol. 4, Issue 1, pp. 3, (2009) (PubMed).

Puverel, Nakatani, Parras, Soussi-Yanicostas: "Prokineticin receptor 2 expression identifies migrating neuroblasts and their subventricular zone transient-amplifying progenitors in adult mice." in: The Journal of comparative neurology, Vol. 512, Issue 2, pp. 232-42, (2009) (PubMed).

Yu, Fotaki, Mason, Price: "Analysis of early ventral telencephalic defects in mice lacking functional Gli3 protein." in: The Journal of comparative neurology, Vol. 512, Issue 5, pp. 613-27, (2009) (PubMed).

Ralston, Chiriboga, Nonaka: "MASH1: a useful marker in differentiating pulmonary small cell carcinoma from Merkel cell carcinoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 21, Issue 11, pp. 1357-62, (2008) (PubMed).

Seki, Namba, Mochizuki, Onodera: "Clustering, migration, and neurite formation of neural precursor cells in the adult rat hippocampus." in: The Journal of comparative neurology, Vol. 502, Issue 2, pp. 275-90, (2007) (PubMed).

Hu, Ippolito, Garabedian, Humphrey, Gordon: "Molecular characterization of a metastatic neuroendocrine cell cancer arising in the prostates of transgenic mice." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44462-74, (2002) (PubMed).

Sommer, Shah, Rao, Anderson: "The cellular function of MASH1 in autonomic neurogenesis." in: Neuron, Vol. 15, Issue 6, pp. 1245-58, (1996) (PubMed).

Johnson, Birren, Saito, Anderson: "DNA binding and transcriptional regulatory activity of mammalian achaete-scute homologous (MASH) proteins revealed by interaction with a muscle-specific enhancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 8, pp. 3596-600, (1992) (PubMed).

Lo, Johnson, Wuenschell, Saito, Anderson: "Mammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells." in: Genes & development, Vol. 5, Issue 9, pp. 1524-37, (1991) (PubMed).

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: MASH1 (ASCL1 Products)

Background: MASH1 and MASH2 (Mammalian achaete-schute Homolog 1 and 2) are basic helixloop-helix transcription factors which are mammalian homologs of the achaete-schute gene family that is required for neuronal development in Drosophila. The bHLH motif is present in many types of transcription factors including E2-2, E47, MyoD and others. MASH1 has been shown to heterodimerize with other bHLH containing transcription factors. MASH1-E12 heteromers bind to and promote transcriptional activation of muscle creatine kinase, a known target for MyoD activation. However, MASH1 appears to play its primary role during early development of the autonomic nervous system. In committed neuronal precursor cells, early expression of MASH1 activates a subset of neuron-specific genes to promote differentiation. Thus null mutations in the MASH1 gene suggest that MASH1 is a valuable marker for investigation of neural development in mammals. MASH1 is observed as an ~34 kDa protein by SDS-PAGE.

Molecular Weight: 34 kDa

Pathways: Dopaminergic Neurogenesis