Cyclin D1 antibody (full length)

Catalog No. ABIN967431

Product Details anti-Cyclin D1 Antibody

Target: Cyclin D1 (CCND1)

Binding Specificity: full length

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant full-length human cyclin D1

Clone: G124-326

Isotype: IgG1

Application Details

Application Notes: D-type cyclins are differentially expressed in distinct cell types, therefore cell types which have been documented to express high levels of a given D-type cyclin are suggested as positive controls. WI-38 human diploid fibroblasts (ATCC CCL 75), and U-118 human glioblastoma cells (ATCC HTB 15) are suggested for detecting cyclin D1. For flow cytometric analysis, T-47D (ATCC HTB 133) and MCF7 (ATCC HTB 22) human breast carcinoma cell lines are suggested as positive controls. G124-326 can also be used for immunohistochemistry of frozen and paraffin-embedded tissue sections (5-20 µg/ml). For immunohistochemistry, normal colon tissue and the HT-29 colon adenocarcinoma cells (ATCC HTB 38) are suggested as positive controls for detecting cyclin D1. Scattered cells are stained, and staining is nuclear and/or cytoplasmic. However, users are cautioned that the differential expression of cyclin D1 remains to be fully characterized in the literature. Shapiro et al (1995) found many positive cells in non-small cell lung cancer and small lung cancer specimens that overexpress cyclin D1. This suggests that they may also be useful as positive controls for immunohistochemical detection of cyclin D1.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1 (CCND1 Products)

Background: Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins contain a conserved amino acid sequence motif, the cyclin box, which allows their binding to cdks to form active complexes that regulate the progression of the cell cycle. The synthesis and degradation of cyclins is tightly controlled in a cell cycle specific manner. Several classes of cyclins (A-E) have been described. Cyclins have been placed into functional groups as follows: Group 1 (cyclins A, B, D1, D2, D3, E and F) functions primarily in cell cycle reguation, Group 2 (cyclins C and H) also plays a role in transcriptional regulation, Group 3 (cyclins G1, G2 and I) may play a role distinct from other cyclins. Specific substrates for cyclin-cdk complexes include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T Ag), tumor suppressor genes (Rb and p53), and others. The D-type cyclins are involved in regulating the passage of mammalian cells through G1. The reduced molecular weights of cyclin D1 is ~36 kD. G124-326 recognizes human cyclin D1 and cross-reacts with the mouse homolog (Cyl1) of human cyclin D1. It does not cross-react with human cyclins D2 and D3. Recombinant full-length human cyclin D1 was used as immunogen. The antibody was originally evaluated by ELISA, western blot analysis and immunohistochemistry of frozen and paraffin-embedded tissue sections.

Molecular Weight: 36 kDa

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling