TNF alpha antibody (AA 115-130)
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- Target See all TNF alpha Antibodies
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Binding Specificity
- AA 115-130
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TNF alpha antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody reacts with TNFa.
- Purification
- Concentrated.
- Immunogen
- Synthetic TNF-alpha 115-130 hexadecapeptide linked to carrier protein.
- Clone
- B428
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product TNF alpha Primary Antibody
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anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody
TNF alpha Reactivity: Human, Mouse, Rat, Rabbit, Horse WB, ELISA, IHC (p), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha Reactivity: Human, Rat IHC, FACS, IF, StM Host: Mouse Monoclonal TNFA-1172 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibodyTNF alpha Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)TNF alpha Reactivity: Human, Pig, Non-Human Primate FACS Host: Mouse Monoclonal MAb11 FITC
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibodyTNF alpha Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal M1-C4 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibodyTNF alpha Reactivity: Mouse WB Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha Reactivity: Mouse WB, IHC, IP, ICC Host: Mouse Monoclonal C1 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 71-234) antibodyTNF alpha Reactivity: Cow WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibodyTNF alpha Reactivity: Human, Mouse, Rat, Pig, Rabbit WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Immunohistochemistry: 1:50-1:100
ELISA 1:500 -1:1000 (this antibody is not purified and can not be used to coat ELISA plates) .
Staining Procedure: This antibody can be used on frozen and formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. - Comment
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Cellular Localization: cytoplasmic and extracellular.
Recommended Positive Control: Prostate cancer - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.3 mg/ml.
- Buffer
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Alternative Name
- Tumor Necrosis Factor Alpha (TNF alpha Products)
- Molecular Weight
- 17 kDa
- Gene ID
- 7124
- Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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