TNF alpha antibody

Catalog No. ABIN967192

Product Details anti-TNF alpha Antibody

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Purified

Immunogen: Recombinant Human TNF-alpha (BioSource company, Cat.No. PHC3013)

Clone: 2A9B9

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Buffer: PBS

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-TNF alpha antibody (ABIN967192)

Reynolds, Ignatowski, Gallant, Spengler: "Amitriptyline administration transforms tumor necrosis factor-alpha regulation of norepinephrine release in the brain." in: Brain research, Vol. 1023, Issue 1, pp. 112-20, (2004) (PubMed).

Dulak, Tomala, Loboda, Józkowicz: "Nitric oxide-dependent synthesis of vascular endothelial growth factor is impaired by high glucose." in: Life sciences, Vol. 75, Issue 21, pp. 2573-86, (2004) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-alpha (TNF alpha Products)

Background: TNF-alpha (tumor necrosis factor alpha) is an important cytokine produced by numerous cell types including neutrophils, activated lymphocytes, macrophages and NK cells. It plays a critical role in inflammatory responses and in apoptosis. TNF-alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF-alpha exists as a multimer of two, three, or five non covalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. Although it has little effect on many cultured normal human cells, TNF-alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF-alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNFalpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Synonyms: DIF, TNFA, TNFSF2, TNF-alpha

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome