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CD8 antibody

Name: CD8 antibody
Brand: antibodies-online
SKU: ABIN965819
Price: 363 USD
Availability: InStock

CD8 Reactivity: Human FACS, IHC (fro), IF, ELISA, IHC (af) Host: Mouse Monoclonal B362 (RIV 11) unconjugated

(3 references)

Catalog No. ABIN965819

Datasheet as PDF

Target See all CD8 Antibodies

CD8
Reactivity
195

64

25

12

12

12

9

9

8

7

6

6

5

5

5

4

4

4

3

1
Human
Host
225

48

36

4
Mouse
Compatible Secondaries
Clonality
276

30

2
Monoclonal
Conjugate
100

48

37

26

13

6

6

5

5

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8 antibody is un-conjugated
Application
275

66

55

50

49

46

29

21

18

17

15

14

8

5

5

4

3

2

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), ELISA, Immunohistochemistry (Acetone-fixed) (IHC (af))

Specificity

This antibody reacts with a 32 kD molecule present on human suppressor/cytotoxic T-cells. These antibodies also react with sinus-lining cells in the human spleen.

Purification

Purified.

Immunogen

Human peripheral lymphocytes.

Clone

B362 (RIV 11)

Isotype

IgG1

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anti-CD8 (CD8) antibody (PE)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PE

anti-CD8 (CD8) (AA 22-187) antibody
CD8 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD8 (CD8) antibody
CD8 Reactivity: Human FACS, IP, CyTOF Host: Mouse Monoclonal MEM-31 unconjugated

anti-CD8 (CD8) antibody (PE)
CD8 Reactivity: Cat FACS Host: Mouse Monoclonal fCD8 PE

anti-CD8 (CD8) antibody
CD8 Reactivity: Human IHC, WB, IP, ELISA Host: Rabbit Monoclonal 3C3 unconjugated Recombinant Antibody

anti-CD8 (CD8) antibody (Biotin)
CD8 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-31 Biotin

anti-CD8 (CD8) antibody (FITC)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 FITC

anti-CD8 (CD8) antibody (PerCP)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PerCP

anti-CD8 (CD8) antibody (APC)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 APC

Application Notes

Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
ELISA: 0.2-1.0 ug/mL.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections.

Comment

Recommended Positive Control: Tonsil and Lymph Node

Restrictions

For Research Use only
Format

Liquid

Concentration

0.4 mg/ml.

Buffer

50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C
Barber, Dasgupta, Schlossman, Trevillyan, Rudd: "The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 9, pp. 3277-81, (1989) (PubMed).

Jonker, Nooij, den Butter, van Lambalgen, Fuccello: "Side effects and immunogenicity of murine lymphocyte-specific monoclonal antibodies in subhuman primates." in: Transplantation, Vol. 45, Issue 4, pp. 677-82, (1988) (PubMed).

Reinherz, Kung, Goldstein, Schlossman: "A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 3, pp. 1301-7, (1980) (PubMed).
Target

CD8

Abstract

CD8 Products

Gene ID

925