CD34 antibody (C-Term)

Catalog No. ABIN965789

Product Details anti-CD34 Antibody

Target: CD34

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Hematopoietic progenitor cell antigen CD34 (CD34) detection. Tested with WB, IHC-P, IHC-F in Human, Mouse, Rat.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human CD34 , identical to the related mouse and rat sequences.< br/>Immunogen was affinity purified.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

References for anti-CD34 antibody (ABIN965789)

Cheng, Baumhueter, Cacalano, Carver-Moore, Thibodeaux, Thomas, Broxmeyer, Cooper, Hague, Moore, Lasky: "Hematopoietic defects in mice lacking the sialomucin CD34." in: Blood, Vol. 87, Issue 2, pp. 479-90, (1996) (PubMed).

Satterthwaite, Burn, Le Beau, Tenen: "Structure of the gene encoding CD34, a human hematopoietic stem cell antigen." in: Genomics, Vol. 12, Issue 4, pp. 788-94, (1992) (PubMed).

Tenen, Satterthwaite, Borson, Simmons, Eddy, Shows: "Chromosome 1 localization of the gene for CD34, a surface antigen of human stem cells." in: Cytogenetics and cell genetics, Vol. 53, Issue 1, pp. 55-7, (1990) (PubMed).

Target Details for CD34

Target: CD34

Alternative Name: Hematopoietic progenitor cell antigen CD34 (CD34 Products)

Synonyms: AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody

Background: CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD. CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30 % of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.