CD22 antibody

Catalog No. ABIN965778

Product Details anti-CD22 Antibody

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts strongly with CD22. CD22 is expressed on in all B cell cytoplasm. It appears as CD19 on the cell surface in developing B cells. It can also be found on the cell surface of most mature sIg+ B cells and on hairy cell and prolymphocytic leukemia cells. On frozen sections, CD22 is found in follicular mantle and marginal zone B cells. There is weak reaction in germinal center B cells. This antibody can be used for typing of leukemia, assigning B cell lineage, and in flow cytometry to identify mature B cells. Also, it can be used to study B cell adhesion and to identify B cell lymphomas.

Purification: Concentrated.

Immunogen: Raji (Burkitt's lymphoma) cells.

Clone: B456

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100 on frozen tissue only.
Flow cytometry: 1:150-1:300.
Staining Procedure: This antibody can be used on frozen tissue only. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Tonsil, Spleen, Thymus, Lymph Node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22 (CD22 Products)

Gene ID: 933