CD19 antibody

Catalog No. ABIN965770

Product Details anti-CD19 Antibody

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: This CD19 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: Ni-NTA purified recombinant human CD19 expressed in E. Coli strain M15.

Purification: Antibodies are purified by protein A affinity chromatography

Isotype: IgG2a

Application Details

Application Notes: Western Blot: Dilution 1: 2000- 5,000
IHC(P): Dilution 1: 100- 1: 400
IHC(F): Dilution 1: 200- 1: 400
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References for anti-CD19 antibody (ABIN965770)

de Rie, Zeijlemaker, von dem Borne: "Inhibition, by vinca alkaloids and colchicine, of antigenic modulation induced by anti-CD19 monoclonal antibodies." in: Leukemia research, Vol. 12, Issue 2, pp. 135-41, (1988) (PubMed).

De Rie, Zeijlemaker, Von dem Borne, Out: "Evaluation of a method of production and purification of monoclonal antibodies for clinical applications." in: Journal of immunological methods, Vol. 102, Issue 2, pp. 187-93, (1987) (PubMed).

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19 (CD19 Products)

Synonyms: B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody

Background: The CD19 antigen (95kDa) is expressed from the earliest stage of B progenitor development, on all peripheral B cells including germinal centre B cells, and all B cell lines and B cell leukaemia tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturation to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.

Gene ID: 930

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway