FITC antibody

Catalog No. ABIN958555

Product Details anti-FITC Antibody

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This FITC antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: FITC. The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. Cross-reactivity This antiserum has not been tested for cross-reactivity with other fluorescent compounds.

Purification: Purified IgG

Immunogen: FITC

Type of Immunogen: Purified protein

Clone: NI 239

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, WB

Usage: This product is intended for use to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e. g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotinavidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile distilled water 0.5 ml

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC (FITC Products)

Target Type: Chemical

Background: Name/Gene ID: FITC

Synonyms: FITC