SYT1 antibody

Catalog No. ABIN955025

Product Details anti-SYT1 Antibody

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Human, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: Recognizes Synaptotagmin-1.

Purification: Affinity Chromatography using a peptide column.

Immunogen: Four different antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (NP_033332) and Human (NP_005630) gene products. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.4 mg/ml. Finally, equal volumes of each of the four affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. Antibodies were affinity-purified and mixed in equal concentrations.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: Synaptotagmin-1 (SYT1 Products)

Background: Human Synaptotagmin-1 is a 47,442 dalton protein (422 amino acids) associated with pre-synaptic terminals of the PNS and CNS. Synaptotagmin-1 binds calcium and initiates the fusion of vesicles with the pre-synaptic membrane. This protein is also believed to be the target of proteolytic activity associated with Botulinum neurotoxin A.Synonyms: SVP65, SYT, SYT1, Synaptotagmin I, Synaptotagmin1, SytI, p65

Gene ID: 6857

NCBI Accession: NP_001129277

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport