STAT1
Reactivity: Human
WB, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Do not freeze.
Storage
4 °C
Storage Comment
Store at 2-8°C. Do not freeze.
Kovarik, Boudny, Kocak, Lauerova, Fait, Vagundova: "Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation." in: International journal of molecular medicine, Vol. 12, Issue 3, pp. 335-40, (2003) (PubMed).
Boudný, Kocák, Lauerová, Kovarík: "Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients." in: Folia biologica, Vol. 49, Issue 4, pp. 142-6, (2003) (PubMed).
Target
STAT1
(Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))
Signal transducer and activator of transcription 1,STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.,CANDF7, IMD31, ISGF3