Kinesin (heavy chain) antibody

Catalog No. ABIN94412

Product Details

Target: Kinesin (heavy chain)

Reactivity: Human, Mouse, Pig, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC)

Specificity: The antibody KN-02 recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.

Cross-Reactivity (Details): Mouse, Human, Rat, Other not tested, Porcine

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Enriched fraction of porcine brain kinesin.

Clone: KN-02

Isotype: IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References

Malcová-Janatová, Richterová, Dráber, Hasek: "Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains." in: Folia microbiologica, Vol. 49, Issue 6, pp. 665-70, (2005) (PubMed).

Mac?rek, Dráberová, Richterová, Böhm, Dráber: "Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin." in: Hybridoma and hybridomics, Vol. 21, Issue 6, pp. 457-62, (2003) (PubMed).

Target Details

Target: Kinesin (heavy chain)

Background: Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.,KHC, KINHC, KIF5

Pathways: Peptide Hormone Metabolism