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- Target See all CD8 Antibodies
- CD8
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD8 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP)
- Specificity
- The antibody MEM-87 recognizes an extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa).
- Cross-Reactivity (Details)
- Human
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- Leukocytes of a patient suffering from LGL Type Leukemia.
- Clone
- MEM-87
- Isotype
- IgG1
- Top Product
- Discover our top product CD8 Primary Antibody
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anti-CD8 (CD8) (AA 51-150) antibody
CD8 Reactivity: Human, Mouse, Rat FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated
anti-CD8 (CD8) antibody (PE)CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PE
anti-CD8 (CD8) (AA 22-187) antibodyCD8 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-CD8 (CD8) antibodyCD8 Reactivity: Human FACS, IP, CyTOF Host: Mouse Monoclonal MEM-31 unconjugated
anti-CD8 (CD8) antibody (PE)CD8 Reactivity: Cat FACS Host: Mouse Monoclonal fCD8 PE
anti-CD8 (CD8) antibodyCD8 Reactivity: Human IHC, IP, WB, ELISA Host: Rabbit Monoclonal 3C3 unconjugated Recombinant Antibody
anti-CD8 (CD8) antibody (Biotin)CD8 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-31 Biotin
anti-CD8 (CD8) antibody (FITC)CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 FITC
anti-CD8 (CD8) antibody (PerCP)CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PerCP
anti-CD8 (CD8) antibody (APC)CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 APC
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- Application Notes
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Flow cytometry: Recommended dilution: 2 μg/mL.
Immunoprecipitation: Excellent for immunoisolation of CD8 positive T cells. - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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- Target
- CD8
- Alternative Name
- CD8 (CD8 Products)
- Synonyms
- CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody
- Background
- The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,p32, LEU2
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