RBCL (Isoform 1) antibody

Catalog No. ABIN93524

Product Details

Target: RBCL

Binding Specificity: Isoform 1

Reactivity: Arabidopsis thaliana, Lobaria pulmonaria, Pisum sativum, Potato, Spinach

Host: Chicken

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)

Purification: affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1

Application Details

Application Notes: Recommended Dilution: 1 : 10 000 - 1 : 20 000 on 2 µg of total cellular protein, detected with standard ECL(WB), (IL) tested on a grass species, formaldehyde-fixed and paraffin-embeddedtissue following the protocol from Gonzalez et al. (1998) Plant Physiol. V. 116.

Comment:

This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For reconstitution add 225 µL of milliQ water per each tube

Concentration: 16 µg/µL

Buffer: PBS pH 8.0+ 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Make aliquots to avoid working with a stock.
Please, Remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubek.

Storage: 4 °C

References

Abdel-Ghany, Pilon: "MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15932-45, (2008) (PubMed).

Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).

Menon, Dou, Heinhorst, Shively, Cannon: "Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species." in: PLoS ONE, Vol. 3, Issue 10, pp. e3570, (2008) (PubMed).

Target Details

Target: RBCL

Background: AGI Code: ATCG00490
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.

Molecular Weight: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)

UniProt: Q3ALL1, O03042