Vimentin antibody
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Vimentin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Immunogen
- Vimentin antibody was raised in Mouse using a purified recombinant fragment of Vimentin expressed in E. coli as the immunogen.
- Clone
- 9E7E7-5G3F10-5G3F10E5
- Isotype
- IgG1
- Top Product
- Discover our top product VIM Primary Antibody
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- Application Notes
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ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as a liquid in ascitic fluid containing 0.03 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C for short term storage. Store at -20 °C for long term storage.
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- Target
- Vimentin (VIM)
- Alternative Name
- Vimentin (VIM Products)
- Background
- Vimentin is a member of the intermediate filament family of proteins that is especially found in connective tissue. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Although most intermediate filaments are stable structures, in fibroblasts, vimentin exists as a dynamic structure. This filament is used as a marker for mesodermally derived tissues, and as such can be used as an immunohistochemical marker for sarcomas. Synonyms: Monoclonal Vimentin antibody, Anti-Vimentin antibody.
- Pathways
- Caspase Cascade in Apoptosis
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