DYKDDDDK Tag antibody (HRP)

Catalog No. ABIN920829

Product Details anti-DYKDDDDK Tag Antibody (HRP)

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DYKDDDDK Tag antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Sequence: DYKDDDDK

Application Details

Application Notes: ELISA: Use at a 1:15,000 - 1:50,000 dilution. Western blot: Use at 0.2 - 1.0 µg/mL. Immunocytochemistry: Use at a 1:200 - 1:500 dilution. Immunofluorescence: Use at 1.0 - 5.0 µg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 mL at 1.0 mg/mL HRP-conjugated polyclonal antibody in 50 mM HEPES, saline with stabilizer and 0.002% thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Alternative Name: Flag Tag (DYKDDDDK Tag Products)

Target Type: Tag

Background: Rabbits were immunized with the DYKDDDDK peptide (DYKDDDDK-epitope) conjugated with KLH. After multiple immunizations in Freund's adjuvant, serum was collected and the antibodies were immunoaffinity-purified using the peptide coupled with a different carrier. The affinity-purified antibodies were then conjugated with horseradish peroxidase (HRP).