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DYKDDDDK Tag antibody (FITC)

WB, ELISA Host: Rabbit Polyclonal FITC
Catalog No. ABIN920828
  • Target See all DYKDDDDK Tag products
    DYKDDDDK Tag
    Reactivity
    Please inquire
    Host
    • 52
    • 48
    • 8
    • 7
    • 6
    Rabbit
    Clonality
    • 61
    • 59
    Polyclonal
    Conjugate
    • 42
    • 14
    • 10
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This DYKDDDDK Tag antibody is conjugated to FITC
    Application
    • 74
    • 59
    • 26
    • 21
    • 19
    • 14
    • 12
    • 12
    • 9
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Sequence
    DYKDDDDK
  • Application Notes
    ELISA: Use at a 1:5,000 - 1:20,000 dilution. Western blot: Use at a 1:1,000 - 1:5,000 dilution.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    0.1 mL at 1.0 mg/mL FITC-conjugated polyclonal antibody in PBS, pH 7.0 with protein stabilizer and 0.1% sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    DYKDDDDK Tag
    Alternative Name
    Flag Tag (DYKDDDDK Tag Products)
    Target Type
    Tag
    Background
    Rabbits were immunized with the DYKDDDDK (DYKDDDDK) peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, serum was collected and the antibodies were affinity-purified using the peptide immobilized on a solid phase. The affinity-purified antibodies were then conjugated with Fluorescein Isothiocyanate (FITC). Prior to conjugation, the antibodies were analyzed by ELISA and Western blot. Specification: The molar F/P ratio is 3.14.
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