FAS antibody (FITC)

Catalog No. ABIN920646

Product Details anti-FAS Antibody (FITC)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Specificity: Reacts specifically with the human Fas antigen, although the epitope has not been mapped. Binds both the soluble and membrane-bound forms of Fas. Does not react with mouse or gibbon Fas. Recognizes activated T-cells and a subpopulation of B-cells. The cell-surface Fas antigen is a 48 kDa transmembrane protein that can mediate apoptosis/programmed cell death and belongs to the nerve growth factor (NGF)/tumor necrosis factor (TNF) receptor superfamily. The human Fas antigen is expressed in various human cells, including activated peripheral T and B lymphocytes, Various tumor cell lines, leukemic T cells, and myeloid cells. It is also differentially expressed on human thymocytes during thymic maturation.

Characteristics: Mouse myeloma (P3.X63.Ag8.653) x immunized mouse (Balb/c) spleen cells.

Purification: Purified by protein A chromatography

Purity: > 95 % by SDS-PAGE.

Immunogen: Human B lymphoblast cell line SKW6.4 cell surface molecules

Clone: APO-1-1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on cryostat sections and on cytospin preparations. Not suitable for use on paraffin sections. Flow Cytometry: Use at 1:20 dilution (as determined on SKW6.4 cells.

Restrictions: For Research Use only

Handling

Buffer: FITC-labeled antibody in 1 ml PBS, pH 7.4 with 1% BSA and 0.02% NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: Fas (FAS Products)

Background: Monoclonal antibody recognizing Fas protein, FITC-labeled for simplified flow cytometry procedures. Clone Apo-1-1 is an IgG1 isotype switch variant of the IgG3 clone APO-1-3 (Cat. No. MC-095).

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity