GFP Tag antibody

Catalog No. ABIN871608

Product Details anti-GFP Tag Antibody

Target: GFP Tag

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP Tag antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: GFP Antibody detects GFP and GFP fusion proteins.

Application Details

Application Notes: Western blotting 1:2000. Immunoprecipitation 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: -20 °C

Target Details for GFP Tag

Target: GFP Tag

Alternative Name: GFP-Tag (GFP Tag Products)

Target Type: Tag

Background: This polyclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coup s ide pled) corresponding to the carboxy terminus EGFP. The green fluorescent prote (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequore victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.