HA-Tag antibody

Catalog No. ABIN871606

Product Details anti-HA-Tag Antibody

Target: HA-Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This HA-Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Sequence: YPYDVPDYA

Specificity: HA-Tag mouse mAb detects over-expressed proteins containing the HA epitope tag.

Application Details

Application Notes: Western blotting 1:5000. Immunofluorescence 1:1000. Immunoprecipitation 1:100. ELISA 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: -20 °C

Target Details for HA-Tag

Target: HA-Tag

Abstract: HA-Tag Products

Target Type: Tag

Background: This monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the infuenza hemagglutinin epitope (YPYDVPDYA) (KLH-coupled). Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allow for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.