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Histone 3 antibody

H3 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN866059

Datasheet as PDF

Target See all Histone 3 (H3) Antibodies

Histone 3 (H3)
Reactivity
1355

928

855

58

41

41

40

40

39

35

34

30

26

22

20

6

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Human
Host
1174

239

8

5
Rabbit
Compatible Secondaries
Clonality
1097

328

1
Polyclonal
Conjugate
794

77

44

44

44

44

44

44

34

33

21

18

18

18

17

17

17

17

17

17

17

17

12

1
This Histone 3 antibody is un-conjugated
Application
1032

405

374

314

239

188

173

166

163

154

139

128

116

48

35

34

32

22

16

14

7

7

6

4

3

1

1

1
Western Blotting (WB)

Specificity

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of Histone H3.

Cross-Reactivity

Human, Mouse (Murine), Rat (Rattus)

Characteristics

Positive Control: HepG2, NIH/3T3, Rat liver.

Purification

Immunogen affinity purified

Isotype

IgG

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H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

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H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone 3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27me) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:1,000-1:2,000

Restrictions

For Research Use only
Format

Liquid

Buffer

1xTBS (pH7.4), 0.5% BSA, 40% Glycerol

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C
Target

Histone 3 (H3)

Alternative Name

Histone H3 (H3 Products)

Synonyms

H-3 antibody, histocompatibility 3 antibody, H3 antibody

Background

The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. This Antibody detects endogenous levels of total histone H3 protein, and does not cross-react with other histones.

Molecular Weight

~17kDa

UniProt

P68431